Objective Survey on ticks and host animals of severe fever with thrombocytopenia syndrome virus (SFTSV) in Huanggang, Hubei province, to provide basic data for the control and prevention of this vector-borne disease. Methods Ticks and animal serum samples were collected from affected areas from April to June in 2013, and SFTSV were detected by Real-time PCR. Results A total of 256 ticks were collected from grassland and livestock, after classification and identification, these collected ticks mostly were classified to 4 species, 4 genus, 1 family, Haemaphysalis longicornis was predominant species. The results of Real-time PCR of ticks, cattle and sheep serum samples showed that infection rates were 3.13%(8/256), 17.65% (3/17), and 12.50% (1/8), respectively. Conclusion Haemaphysalis longicornis was predominant species in Huanggang, the results of Real-time PCR indicated that the SFTS may transmitted by “host animals-to-ticks-to-human”, avoid tick biting is the key to prevent this disease.

Objective The purpose of this paper was to evaluate the efficacy of 0.004% fipronil bait against Reticulitermes in the laboratory and the sites where Reticulitermes occurred. Methods Efficacy test of fipronil bait against Reticulitermes chinensis was conducted in the laboratory and efficacy test against R. chinensis and R. flaviceps of was conducted in the buildings where Reticulitermes occurred. Results In the laboratory, the death of R. flaviceps occurred massively after feeding with bait in three days. The average mortality rate reached to 78.00%. All of the workers and soldiers were dead in 8 d. The average feeding rate of baits was 5.15%. For the application studies, fipronil bait was used in 15 office buildings and residences for R. chinensis and R. flaviceps from 2009 to 2015. Reticulitermes were wiped out by a single application for 13 sites, and twice, three times for the other 2 sites, respectively. Conclusion Reticulitermes chinensis and R. flaviceps could be effectively controlled by 0.004% fipronil bait in the sites where they occur.

Objective In this study, we investigated the population densities and resistance to commonly used insecticides of Culex pipiens quinquefasciatus in Wuhan, Hubei province. Our results can provide new insights into field mosquito control operations. Methods According to GB/T 23797-2009, lamp traps were used to caputre mosquitoes during March to November in 2014. The larve impregnation method which is recommended by WHO, was used to determine the susceptibility of larve (LC₅₀ with 95%CI) to deltamethrin, DDVP, DDT, propoxur and temephos. Results The population densities of C_x. pipiens quinquefasciatus throughout the year showed a single peak between May and June, the average density in stables was significantly higher than any other habitats. The LC₅₀ values of deltamethrin, DDVP, DDT, propoxur and temephos of C_x. pipiens quinquefasciatus were 0.071 7, 0.469 9, 0.766 0, 0.941 0 and 0.000 78 mg/L, and the resistance ratio were 358.50, 151.14, 42.56,9.72 and 1.30, respectively. Conclusion Our data showed that the peak population densities of adult C_x. pipiens quinquefasciatus was between May and June in Wuhan, it is also the key month for mosquito control. Considering the prevalence of resistance to chemical insecticides among mosquitoes, integrated management should be promoted to delay the development of resistance.

Objective To observe the control effects of 10% boric acid gel bait on cockroaches in the laboratory and field and compare it with residual spray in killing cockroaches. Methods The killing effects of 10% boric acid gel bait and residual spray were observed in the laboratory and simulated field according to the national standards GB/T 17917.7-2009 and GB/T 17917.10-2009. The decrease in cockroach density was determined by visual measurement after residual spray and application of gel bait in the field, and the killing effect was evaluated accordingly. Results In the laboratory and simulated field, all Blattella germanica died 2 d after treatment. In the field, the average density of B. germanica was 169.25/15 min before residual spray; the density decreased by 9.60% and 6.29% in the first and second weeks after residual spray of 10% cis-cypermethrin suspoemulsion plus 80% DDVP; then, residual spray of 10% cis-cypermethrin emulsion plus 20% propoxur EC was performed, and the density decreased by 6.63% and 5.51% in the third and fourth weeks. Finally, 10% boric acid gel was used, and the average density of B. germanica decreased by 94.10% and 100% in the first and second weeks after treatment. The average density of B. germanica was 1.38/15 min 1 month later. Conclusion The B. germanica population with high insecticide resistance is difficult to be control by residual spray, but 10% boric acid gel bait has good control effect on cockroaches and causes little insecticide resistance. Gel bait is recommendable for cockroach control.

【Abstract】 Objective To learn the resistance situation of Musca domestica from different areas in Hubei province to common insecticides and study its control measures.  Methods Topical application was used in this study.  Results The surveillance results indicated the average LD50 values of DDVP to housefly from different areas was 0.2256, 0.1908 and 0.4428 μg/housefly from 2005 to 2007, respectively. The average LD50 of alpha?cypermethrin was 0.0841 and 0.1102 μg/housefly in 2006 and 2007, respectively. The average LD50 of deltamethrin was 0.0371 and 0.0825 μg/housefly in 2005 and 2007, respectively. The average LD50 of etramethrin in 2005 and 2006 was 0.3952 and 0.2894 μg/housefly, respectively. The average LD50 of acetophenate in 2005 and 2007 was 0.3264 and 0. 3487 μg/housefly. Conclusion M.domestica had high resistance to organic phosphorus and pyrethrum insecticide in Hubei province. No obvious resistance against acetophenate had been found. The mixed and rotation use of insecticides contributed to the control the resistance development of M.domestica.